Hello,
I wish to MAP RNAseq contigs for transcriptomics analysis of a fungus Podosphaera xanthii, could you please add it to list of reference genomes? Thank you.
Hi @LaurenceBindsch,
In Galaxy, all mapping tools, such as HiSAT2, support custom reference genomes. Upload the reference genome in FASTA format into your Galaxy account and in job setup change source of genome from built-in to From History or something similar. The aligner indexes the ref genome before mapping, so no other action is required. Make sure you also download the gene annotation file for the reference genome.
Hope that helps.
Kind regards,
Igor
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