ATAC-seq macs2 peak splitting in sliding windows

I used macs2 to call peaks for atac-seq data. now my goal is to split the peaks into 50 bp windows with 25 bp steps and then calculate the Tn5 integration frequency in each window.

How should I proceed with that?

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Welcome @nancy

Sorry for the delayed reply. Try reviewing your methods to those in the GTN tutorial that covers ATAC-seq analysis: