ChiRa for Chimeric eClip data processing - Split ChiRa collapse out put as files to large processing

usegalaxy.org.au support
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Hi there,

I’m using the ChiRA workflow from "RNA-RNA interactome data analysis" transcriptomics tutorial.

And I have had to split my ChiRa collapse output files into 500k read fasta files to enable mapping; as the original files were exceeding the allowed memory.

Will this cause a downstream issue with the workflow? As single samples are split across 5 to up to 32 fasta files.

What steps would you advise to merge the outputs, and at what stage would you merge them?

If I am planning to only look at the Chimeric reads, would this make a difference?

Appreciate your help!

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Hi @S_Staplet,

The tutorial is marked as “possibly supported” on Galaxy Australia. Please, report the failed jobs to the server admins using the following procedure: click at any output from a failed, click at Error icon, the one looking like lady bird beetle, at the bottom of the middle window click Report button. Do not delete output(s) from a failed job when you report the error to admins.

Thank you!

Igor