differential gene expression analysis by denovo data

Dear All
how to perform differential gene expression from 4 transcriptomic library a two from control and 2 from treated which are uploaded on NCBI. So need to do the differential gene expression for fpkm/rpkm value so to check out the expression of identified element. Anybody can help.
highly thank ful to you

Hi @Anand_Mishra,
if a reference genome and a gene annotation is available, please check Galaxy Training!
If the genome assembly is not available, assemble transcription with Trinity or rnaSPAdes and follow any transcriptome based protocol.
Hope this helps.
Kind regards,

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