Error with Unique.seq

I am following the instruction of 16S Microbial Analysis with mothur (extended) and using the example data. Everything went well until the step of using Unique.seqs. I have attached the parameter that I used and the error messages. Can anyone please let me know what did I do wrong? Thank you!

1 Like

Status resolved: Troublshooting Unique.seqs: Status: resolved, please rerun prior failures

Welcome, @diegozhu

This tool has a small bug right now. Please see the solution on this topic for the current options.

Thanks! I try the and it works!

1 Like