Error with Unique.seq

[diegozhu]

Hi,
I am following the instruction of 16S Microbial Analysis with mothur (extended) and using the example data. Everything went well until the step of using Unique.seqs. I have attached the parameter that I used and the error messages. Can anyone please let me know what did I do wrong? Thank you!

[jennaj]

Welcome, @diegozhu

This tool has a small bug right now. Please see the solution on this topic for the current options.

Troublshooting Unique.seqs

Jennaj, I am running into problems with the Unique.seq tool in Galaxy. I use Galaxy online to teach a course and we use the 16S Metagenomics (Mouse) dataset as the training module. I have been using this approach for a couple of years now, but we have run into a Fatal Error when running the first step of the Unique.seq tool. We have tried to run Unique.seqs from multiple accounts (students and mine), but always using Galaxy online. I have tried it from our computers at work (faster internet) …

[diegozhu]

Thanks! I try the galaxy.org.au and it works!