I am following the instruction of 16S Microbial Analysis with mothur (extended) and using the example data. Everything went well until the step of using Unique.seqs. I have attached the parameter that I used and the error messages. Can anyone please let me know what did I do wrong? Thank you!
This tool has a small bug right now. Please see the solution on this topic for the current options.
Thanks! I try the galaxy.org.au and it works!