Hi - Is your goal to explore variation?
If so, please review the tools below and see these Galaxy tutorials for examples of complete analysis workflows that include intermediate tools.
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Common tools to use from the step you are at now can be reviewed/used at Public Galaxy servers such as Galaxy Main https://usegalaxy.org, Galaxy EU https://usegalaxy.eu, and Galaxy AU https://usegalaxy.org.au (All Public Galaxy servers: Galaxy Platform Directory: Servers, Clouds, and Deployable Resources - Galaxy Community Hub). Try searching for tools by these keywords: pileup or variant or vcf.
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The Galaxy ToolShed Galaxy | Tool Shed can also be searched for tools that can be used in your own Galaxy instance https://galaxyproject.github.io/.
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GTN Tutorials: Variant Analysis
- More about how/where to use Galaxy GTN tutorials and the included tools/workflows for your own work.
Thanks!