Hello, I’ve been using Limma for differential expression. It had been working pretty well. I’ve been using it with the TREAT method in my analysis. However, I ran some new comparisons on data that had previously worked for a different comparison, but this time it seemed to get an error at the TREAT stage. I have re-ran this comparison without the TREAT setting and the analysis finishes. I’ve also tested a pre-run comparison that previously worked with TREAT but this time it failed. Why might this be happening?