Limma "Run Tool" Not Working

usegalaxy.org support
1

Dear to whom it may concern,

Hello! I hope this message finds you well.

I am currently trying to use the Limma (limma-voom) tool for differential gene expression but for some reason, once I’ve attached the single count matrix file and click “Run Tool”, it’ll briefly say “Please wait” then reset. Is there anyway to troubleshoot this? The version is currently: (Galaxy Version 3.58.1+galaxy0).

Best,

Shawn

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Welcome, @Shawn_Dinh

You probably need to tell the tool which samples are included per factor level. This tutorial has an example → Hands-on: 2: RNA-seq counts to genes / Transcriptomics

Hope that helps! :slight_smile:

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Hi @jennaj, thank you so much for your response and attaching the tutorial. I’ll be sure to look into it!

Best,

Shawn

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