Hi
I am into The Global Picture tutorial Hands-on: Analyses of metagenomics data - The global picture / Analyses of metagenomics data - The global picture / Microbiome. The format from the BIOM1 file generated is 0.9.1 which is no longer accepted. I have tried formatting it in biom-format but still cannot be validated. Any suggestions please. Thanks
Welcome, @yogesh_parmessur
These protocols are newer, so let’s bring in the people who work on these to help more.
- The link to the chat is at the top of the training domain homepage here → Microbiome / Tutorial List
- Then I just cross-posted there, and this is the link to my post. They usually reply back here, but feel free to join the chat, too. → You're invited to talk on Matrix
What would help is if you could clarify more details about where you are working and the tool versions involved. This is best done by sharing your working history. The banner topic at this forum explains how to generate that link, also here → How to get faster help with your question. You can copy and paste that link back here in a reply.
Let’s start there, thanks!
I guess the problem is BIOM v1 format and phinc only accepts BIOM v2 (FYI in galaxy the datatypes are called biom1 and biom2)?
If this is the case one could try to convert it:
Or maybe better: Galaxy
Would be great if you could let us know if this was the problem. Then we should disable phinch for biom1 datasets and update the tutorial.
Dear All
Thanks for the update. Really appreciate.
Finally I worked it out on Friday. We need to create a metadata file and upload it at the make.biom command. This will create 3 files The Anguil and Pampa will fail when considered individually while the collection will produce the Biom1 file. This biom1 file can be saved and uploaded on Phinch. it will work fine.
May be we should edit the tutorial accordingly.
BTW, I used galaxy.org.au and it worked fine.
Thanks and regards
Yogesh