I have been trying to create a protein database from RNA-seq files on the usegalaxy.org server. There are a few tools required for this workflow that are missing on the server. I tried using the EU server where the tools are present but the upload has been taking ages.
I also tried to create a local galaxy server but since I have Windows 10, I have been getting one error after another.
My last option is to try a dual boot but I wanted to check if there is an easier way of getting the tools added to the usegalaxy.org server. The tools are: 1 CustomProDB, 2.Convert gffCompare annotated GTF to BED, 3. Translate BED transcripts cDNA in 3frames or CDS, 4. bed to protein map, and others under Creating FASTA Databases mentioned here- https://training.galaxyproject.org/training-material/topics/proteomics/tutorials/proteogenomics-dbcreation/tutorial.html#translate-transcripts
Also, if anyone knows any other way to create a local Galaxy server on Windows 10 apart from using the dual boot method, I would appreciate the information (I have tried using python 3.6 and Ubuntu but it did not work for me).
Thank you and Happy Holidays