Problems regarding file upload in DeSeq2

usegalaxy.org support
,
1

My count file collection is not appearing in the “Input” section whenever I am selecting the option “Select group tags corresponding to levels”. I would you to resolve the issue as soon as possible.

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Thank you

2

Hi @Sukanya_Ghosh,

It seems you have individual count files in the history. Try DESeq2 with the default option, Select datasets per level.

Check DESeq2 job setup in this tutorial:

Hope that helps.

Kind regards,
Igor

3

Whenever I am running the operation with the option “Select datasets per level”, I am receiving an error message as follows.

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Please help me resolve this issue.
Thank you in advance

4

Hi @Sukanya_Ghosh
Do you have replicates? DESeq2 requires replicates for reliable statistics. This error message appears when no replicates are present. For two conditions you need at least three samples, preferably more.
Kind regards,
Igor

5

Okk I got it…thanks a lot

6

Hi @Sukanya_Ghosh,

More info on the topic here explaining why replicates are required:

Kind regards,
Igor

7

Thank you so much