hi, I’m trying to do a heatmap in galaxy using the normalized data that I previously filtered with a p-adj >0.05, for that, I’m using the heatmap2 tool but when I visualize the heat map the samples with treatment are on the left and the controls are on the right, how can I fix this, so that the controls are on the left and the treatments are on the right.
For references the configuration that was used is:
- Data transformation: plot data as it is
- Enable data clustering: yes
- Clustering columns and rows: cluster rows and columns
- Distance method: euclidean
- Clustering method: complete
- Labeling columns and rows: label columns and not rows
- Coloring groups: blue to white to red
- Data scaling: scale my data by row
For context, I’m trying to visualize how the samples behave in general by condition (the columns are the different conditions (3 with treatment and 3 controls) and the rows are the genes)