Dear Galaxy staff,
I am trying to do a 16S amplicon analysis using QIIME2 on Galaxy following the instructions here: Moving Pictures tutorial 🎥 - Microbiome marker gene analysis with QIIME 2. Intriguingly, I found the latest version of QIIME2 installed on Galaxy (2025.4.0+q2galaxy.2025.4.0 on https://usegalaxy.org/) failed constantly, but the same analysis could be done using an earlier version (2024.10.0+q2galaxy.2024.10.0). The same is true for several other QIIME2 tools.
In addition, the tool “qiime2 composition da-barplot” is mentioned in the tutorial, but could not be found on Galaxy. There is an earlier discussion on it (da-barplot for Qiime2 visuaisation of ANCOM-BC), but it seems that the tool has not been installed yet.
Could you please take a look at these two issues? Thanks very much!
Best,
Xiaofan