Hello, I attempted to input two WGS data sets (two different sets of reads) and a reference genome from NCBI into the BWA-MEM2 tool. The job failed and resulting in the following messages: Execution resulted in the following messages: Fatal error: Exit code 1 () Tool generated the following stan…

Hi @edf393 The FAQs explain what you can do in general to continue working at a public Galaxy server. In short: use an indexed genome as the mapping target (bosTau8 instead) + split the query reads into a collection, run some QA, map, then merge the results. Or, you can consider setting up a priva…

BWA-MEM2 on data 1, data 8, and data 5 (mapped reads in BAM format) Dbkey: bosTau9 Format: bam Galaxy Tool ID: toolshed.g2.bx.psu.edu/repos/iuc/bwa_mem2/bwa_mem2/2.2.1+galaxy0 Tool Parameters Will you select a reference genome from your history or use a built-in index? History Use the followi…

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Troubleshooting an error (exit code 1) when performing BWA-MEM2 on WGS data

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