Uploading file to Galaxy with Bioblend not working

UPDATE: I originally posted before Christmas but my posts now has updates:

  • reflecting the use of the original Bioblend API vs the OO BioBlend API).
  • with details about manual upload to Galaxy GUI

I am trying to use BioBlend to upload a file (locally stored on my computer). I have tried doing so in 2 ways:
(1) Using the original Bioblend API
(2) Using the OO BioBlend API

In both cases, I obtain the same error (at least it seems). Here is my code:

Using original BioBlend API (bioblend.galaxy)

import os
from bioblend.galaxy import GalaxyInstance
from bioblend.galaxy.histories import HistoryClient
from bioblend.galaxy.tools import ToolClient


# Set up GalaxyInstance ####
gi = GalaxyInstance("https://usegalaxy.org", "my-instance-number")

# Make a new history ####
historyClient = HistoryClient(gi)
hi = historyClient.create_history("tmp2-100119")

# Upload datasets ####
toolClient = ToolClient(gi)
counts = toolClient.upload_file("example.txt", hi["id"])
counts2 = toolClient.upload_file("counted.txt",hi["id"]) # not working

Using OO BioBlend API (bioblend.galaxy.objects)

import os
from bioblend.galaxy.objects import GalaxyInstance


# Set up GalaxyInstance ####
gi = GalaxyInstance("https://usegalaxy.org", "my-instance-number")

# Make a new history ####
hi = gi.histories.create("tmp-100119")

# Upload datasets ####
counts = hi.upload_file("example.txt")
counts2 = hi.upload_file("counted.txt", file_type="tabular") # not working

In both code version, I have the same problem: I can upload a simple file called space-delimited example.txt which looks like this below. On the Galaxy GUI, I can see this file and it says “format: txt” and “uploaded txt file”.

1 2 3

However, I cannot upload my counted.txt file. This file is a tab delimited file, which has 51,827 lines. It represents a matrix of counts I want to perform DE analysis on. It looks like so (first few lines):

GeneID	S1	S2	S3	S4	S5	S6	S7	S8	S9
gene1    0	 1	 0	 2	 0	 1	 0	13      34
gene2	 4	 0	 0	 3	 0	 5	 0	 1      13
gene2	13	44	94	30      45	34	46	40	44
gene3	 2	 0	 0	 0	 0	 0	 0	 5       6

The error I get in my terminal is as follows (the upload_file commands takes a min or so before the error appears). There are slightly different for both codes but they both in a ConnectionError: (‘Connection aborted.’, RemoteDisconnected(‘Remote end closed connection without response’,)) error.

Traceback (most recent call last):

  File "<ipython-input-14-345175af4c4e>", line 1, in <module>
counts2 = hi.upload_file("counted.txt", file_type="tabular") # not working

File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/bioblend/galaxy/objects/wrappers.py", line 1000, in upload_file
out_dict = self.gi.gi.tools.upload_file(path, self.id, **kwargs)

File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/bioblend/galaxy/tools/__init__.py", line 162, in upload_file
return self._tool_post(payload, files_attached=True)

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/bioblend/galaxy/tools/__init__.py", line 232, in _tool_post
return self._post(payload, files_attached=files_attached)

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/bioblend/galaxy/client.py", line 152, in _post

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/bioblend/galaxyclient.py", line 137, in make_post_request

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/requests/api.py", line 116, in post
return request('post', url, data=data, json=json, **kwargs)

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/requests/api.py", line 60, in request
return session.request(method=method, url=url, **kwargs)

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/requests/sessions.py", line 533, in request
resp = self.send(prep, **send_kwargs)

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/requests/sessions.py", line 646, in send
r = adapter.send(request, **kwargs)

 File "/home/username/anaconda3/envs/myenv-3.6/lib/python3.6/site-packages/requests/adapters.py", line 498, in send
raise ConnectionError(err, request=request)

ConnectionError: ('Connection aborted.', RemoteDisconnected('Remote end closed connection without response',))

I have managed to upload this counted.txt file to the Galaxy GUI successfully. It says “format: tabular” and “uploaded tabular file”. I have even manage to run some analyses on it. This file is 2.1 MB in size.

I am very lost as to what is causing this error. Any help would be deeply appreciated.

1 Like

How large is the file?

Does it contain unusual content (HTML, XML)?

What happens when you try to load it with the Upload tool through the GUI. Does it load Ok? Do you have to use FTP first because of the size? Does “autodetect” for format detect what you would expect?

Hi @jennaj, apologies for the delay in my response. I have updated my post and given more details. Hopefully that should answer your questions. I suspect something is wrong in the sense that BioBlend can’t recognize my file type but even when I tell it what type it is (tabular), the error remains. Many thanks.

This has been later reported on https://github.com/galaxyproject/bioblend/issues/271 and solved in the BioBlend master branch.