Hello everyone! I’m a newer in bioinformatic and galaxy - sorry
I trying to use RNA STAR tool. I have full human transcriptom (RNAseq) in 8files (with R1 and R2 reads). So I have to get names of genes and their characteristics (pathogenic etc) in the end for clinicians.
I want to create vcf file for 1-snpsift variant type and 2-snpsift annotate SNPs from dbsnp. And after this I wanted download this vcf file (for ClinVar annotation etc).
I dont need the Trimmomatic so I was trying to do next:
Fastq–>RNA-STAR(hg38)–>Samtoolsmerge(for mapped.bam)—>FreeBayes
But FreeBayes show me the error:
- { “code_desc”: “”, “desc”: “Fatal error: Exit code 101 ()”, “error_level”: 3, “exit_code”: 101, “type”: “exit_code” }
Job API ID: bbd44e69cb8906b5339b2fad2d9562d3
Please explain me what I do wrong and which tools I need to use for my purpose?