Hello everyone, I wanted to use Deseq 2 and till now I used the SRA data for mapping and Quality check but I’m not able to do that for Deseq2, can anyone suggest which data to take and how to get that data in Galaxy.
it depends on your approach; did you mapped your reads against the genome (e.g. STAR or HISAT2) or did you perform a quasi-mapping approach (e.g. Salmon or Kallisto). I recommend you to have a look at the transcriptomics training material.
Hello Sir, I mapped my reads against the genome using Rna sta, so how do i proceed further and also could you enlighten me if i’m using quasi approach as well?.