Help with DeSEQ Analysis

rna-seq
deseq2

#1

Hello all,

I’m relatively new to bioinformatics having been trained primarily as a molecular biologist, and am using Galaxy at the recommendation of a colleague due to my limited programming ability. I am trying to use the DeSEQ tool to statistically analyze the differentially expressed genes in my RNA-seq data set. However, I am unsure of how to convert my Excel datasheet into a suitable file for Galaxy analysis, and how to setup the correct parameters. Would someone kindly be able to guide me through the process?


#2

Have you seen https://galaxyproject.org/tutorials/g101/ and https://galaxyproject.github.io/training-material/ ?