Hi, I’m using a copy of the workflow that was constructed from the history ‘Human Whole-Exome Sequences for three individuals sequenced’, which was shared by user abdalrahman_alalwan in this workflow.
Once the process is complete, a VCF file is generated. At this point, what analyses should be performed to further understand the data.
Hi @baibaiya
I think I know which workflow you are referring to, but you can clarify that with a share link or the server URL and search terms to use against published workflows.
This tutorial is one place to start. Hands-on: Exome sequencing data analysis for diagnosing a genetic disease / Exome sequencing data analysis for diagnosing a genetic disease / Variant Analysis. See near the end where the data is explored using Gemini. Once loaded, you can design custom searches, too, not just those in the examples.
Hope this helps! 
HI jennaj
Galaxy this is my use of the working process of the connection. It was a pedigree whole exome sequencing analysis.
Thank you so much for your assistance.
Wish you everything goes well.
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Great, thanks for confirming the workflow @baibaiya
That person was either following the tutorial I linked above, or doing something else very similar but simplified for a class. If you want a more “polished” workflow, you can review the one included in the tutorial.
But the main point: the preprocessing has been done, and the result is a VCF file with calls for multiple samples. My original suggestion is one way to explore, and the other variant tutorials will have more.
HI jennaj
I want to analyze the whole exome sequencing analysis of non-genetic diseases. Is there any good tutorial to recommend?
Thank you so much for your assistance.
Wish you everything goes well.