I’m trying to replicate the results of the scRNAseq dataset from the following publication “Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq” from this link https://science-sciencemag-org.ezp-prod1.hul.harvard.edu/content/352/6282/189. They have provided a series matrix at GEO “GEO Accession viewer”. I would like advice from the forum on the best Galaxy tools to format this file for analysis.
I thought this file looked like a a processed dataset (like what we would get after RNA-Star Solo pre-processing), so I started out by putting this directly into the RACEID Initial Processing to make a Count Matrix (RDS). However this didn’t work. When I tried to plug this directly into the RACEID clustering directly, I also got an error.
What are the proper steps and tools to process a series matrix into a matrix I can put it into RACEID for analysis? Thanks for your help!