Collection folder VIDEO: How to create a single or paired end dataset collection folder (shapes and organization)

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1

I need help. I’m trying to use Metawrap on usegalaxy.eu to analyze binning files. After entering the binning files, it asks for the original files prior to the assembly. This is required information, but there’s no option to add the file. How can I use this analysis step in Metawrap without it allowing me to add the necessary files?

I thank you in advance for your help

2

Welcome @Bioinfoufgd

Hopefully I can explain a bit about the usage! You’ll need to include some reads files since what this tool is doing is generating statistics about your specific reads versus your assembly.

I created a very simple demonstration history here that you can review or import to play around with to see how it is all organized.

This is a screenshot of the tool form with the inputs from the history selected.

Screenshot 2025-09-24 at 4.10.48 PM
Screenshot 2025-09-24 at 4.10.48 PM1650×914 95.9 KB

  1. The assembly in a single file
  2. The reads are each in their own single file, then they are combined into a paired-end collection folder. The tool is expecting this folder shape and will not accept the read files just as individual datasets.

I’m guessing that you might still need to organize the reads into a paired end collection folder.

Here is a little movie showing how to do it. Let us know if it helps! :slight_smile:

3

Hi Jennaj,

Thank you so much for your help! Your explanations were perfect, and I was able to run metaWRAP successfully!!

Best regards,

Rodrigo

4

Great! Thanks for letting us know :rocket: