I have been attempting for HOURS to use DESeq2, and I keep running into the error message
Error in .rowNamesDF<-(x, value = value) : invalid ‘row.names’ length
Calls: rownames<- … row.names<- → row.names<-.data.frame → .rowNamesDF<-
I have tried the data with and without headings, filtering low-count genes, using gene symbols vs ensembl identifiers, sorting data in EXCEL to see if there’s blanks… I’m stumped! HELP! I’ve successfully used DESeq2 previously, so I’m extra stumped.
P.S. I’d share more details but it’s not my data to share
Hi @RebeccaW,
The error message indicates on a potential job setup/data issue. Make sure you use data type and header settings appropriate for the data, factor names start with a letter, not a digit. Maybe validate your settings using tutorial data from GTN. Usually these files are small, so test should not take much time.
You can report an error to the server support, but this will share the data with the server admins. Alternatively, you can copy several samples into a new history, chage names and provide access to the shared history, so people can check what is going on.