Hi, I have mRNA seq data for 6-time points. I have 150 cells and 2000 genes and for each time point an mRNA matrix. I would like to find the differential gene expressions for each time point and the rest of time points. I would like to use diffxpy. But I have a problem with converting these frames to annoData frame. I put the obs as the name of cells, var as the name of genes but I do not know how put each time point matrix in X matrix there? Does anyone have experience with this topic? Any help is appreciated.
Related Topics
Topic | Replies | Views | Activity | |
---|---|---|---|---|
Differential Expression from Salmon Quant outputs without GTF/GFF3 annotation file | 3 | 464 | November 16, 2022 | |
miRNA differential expression | 0 | 405 | January 5, 2020 | |
differential gene expression analysis by denovo data | 1 | 289 | November 24, 2022 | |
Deseq normalized values and RNA seq analysis
|
0 | 264 | April 1, 2020 | |
DESEq2 error while running | 4 | 248 | October 6, 2023 |