Differential gene expression

Hi, I have mRNA seq data for 6-time points. I have 150 cells and 2000 genes and for each time point an mRNA matrix. I would like to find the differential gene expressions for each time point and the rest of time points. I would like to use diffxpy. But I have a problem with converting these frames to annoData frame. I put the obs as the name of cells, var as the name of genes but I do not know how put each time point matrix in X matrix there? Does anyone have experience with this topic? Any help is appreciated.