Our lab uses Trinity for most of our assembly work, but lately, I’ve been unable to get the tool to run correctly.
What I am attempting to assemble is a set of paired-end Illumina reads, with a file size of about 360MB for each strand. This created a near-instantaneous error report when the job transitioned from ‘pending’ to ‘running’. The job API ID is bbd44e69cb8906b567f21c7489016a08, and the error code is “[Errno 122] Disk quota exceeded”. My understanding of that error code is that it is a memory issue, but we’ve assembled larger datasets than that in the last without issue. Any clarification you could give would be appreciated.