Hi @andre.sa
Try uncompressing the fastq reads before running Trinity
.
Pencil icon > Convert > uncompress. Do this on each output from the Fastq Splitter
tool or on the original dataset 8, then split.
This post has more details about general usage help for Trinity including tutorial links: too much time for start a job? Troubleshooting Trinity inputs + QA/QC steps for RNA-seq assembly - #4 by jennaj
Thanks!