My variant calling jobs are giving errors. I am posting the error messages as snaps. Can someone please help me out with the problem?
Hello,
The tool is expecting a tag that is not present in the input BAM dataset. Specifically, the “sample” attribute.
This information can be added when mapping (read group options) or adjusted after mapping with a tool like AddOrReplaceReadGroups add or replaces read group information.
More help:
- Search the
BAMformat specification PDF with the keyword “sample” to learn more about the attribute: https://samtools.github.io/hts-specs/SAMv1.pdf
*GATK4workflow documentation may also help: https://gatk.broadinstitute.org/hc/en-us - The tool form itself has additional usage/formatting help.
- Tutorials: Galaxy Training!