Fastp - Collection

Hi, i have 2 reads file in my history (R1 and R2) and i want to use the tool fastp to do trimming operations. The only way to give in input a paired files is via collection but when i build it in my history from my 2 files the input box of fastp doesn’t take it. I don’t understans how to produce a right collection from my 2 files that will be suitable for fastp. Thanks

Hi @AleMoxy
You need a list of datasets pairs. The procedure: tick Select items at the left end of grey bar above datasets in history, select two datasets, in pull-down menu with “two out of xxxx selected” select Build list of datasets pairs. Now, complicated part. Depending on file names, Galaxy can pair some files automatically, but not all files. If it does not pair files automatically, provide text string for left (forward) and right (reverse) datasets and click Pair these datasets. Give name to the collection. You can hide the original files, but in your situation I’d rather not hide them.

Hope that helps.

Kind regards,
Igor

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