I am working on CHIPseq on usegalaxy.org.
When I tried to annotate and visualize the data from MACS,
I got a fatal error.
As shown here:
Import genomic features from the file as a GRanges object … OK
Prepare the ‘metadata’ data frame … OK
Make the TxDb object … OK
Error: subscript contains invalid names
And here is the information from Tool Standard Output:
preparing features information… 2020-12-16 06:53:08 AM
identifying nearest features… 2020-12-16 06:53:09 AM
calculating distance from peak to TSS… 2020-12-16 06:53:10 AM
assigning genomic annotation… 2020-12-16 06:53:10 AM
assigning chromosome lengths 2020-12-16 06:53:28 AM
done… 2020-12-16 06:53:28 AM
Is there anything I may do wrong or input inappropriate files to cause this result?
Thanks for your attention to this question.
I will be glad to have your responses.
Szu Shuo Lee