Hi there,
I’m try to analyse two metagenomics sample for V3/V4 regions. I am using a silva database which I used last year for another analysis. Its size is 584mb and it has 11000 sequences in it.
When i run the align.seqs tool it gives the error below. However it works fine with the database silvav4 that used in the tutorial. I’ve read many forum entries but did not see any satisfactory answers. I must solve this issue. Helps are appreciated.
Thank you
Here is the error bug code:
data/jwd05e/main/076/937/76937000/tool_script.sh: line 23: 18 Done echo ‘align.seqs( fasta=fasta.dat, reference=alignment.reference.dat, align=needleman, ksize=8, processors=’${GALAXY_SLOTS:-8}’ )’
19 | sed ‘s/ //g’
20 File size limit exceeded(core dumped) | ./mothur
21 | tee mothur.out.log