I am trying to upload a database of all bacterial nucleotide sequences (gigantic file - 107 GB). I am using FTP via FileZilla, and it got to 80.5 Gb before giving the error “552 Transfer aborted. File too large”. I read something about a 250Gb limit but I think I should be within that - even with files already on Galaxy, it says I am only using 2.7Gb. Not sure what to do (I am definitely no bioinformatician and it took me two weeks to figure out how to download this file -_-). If there’s an alternative to use a database like this on Galaxy that is already uploaded I am all ears. Below is the full set of messages that keep iterating until I stop the queue.
| Error: | File transfer failed after transferring 294,912 bytes in 1 second |
|---|---|
| Status: | Starting upload of /Volumes/MBLab/Bacterial Nucleotide Database - October 2020.fasta |
| Status: | Retrieving directory listing of “/”… |
| Command: | PASV |
| Response: | 227 Entering Passive Mode (129,114,60,56,119,69). |
| Command: | REST 0 |
| Response: | 350 Restarting at 0. Send STORE or RETRIEVE to initiate transfer |
| Command: | MLSD |
| Response: | 150 Opening BINARY mode data connection for MLSD |
| Response: | 226 Transfer complete |
| Command: | PASV |
| Response: | 227 Entering Passive Mode (129,114,60,56,120,177). |
| Command: | REST 80530636800 |
| Response: | 350 Restarting at 80530636800. Send STORE or RETRIEVE to initiate transfer |
| Command: | STOR Bacterial Nucleotide Database - October 2020.fasta |
| Response: | 150 Opening BINARY mode data connection for Bacterial Nucleotide Database - October 2020.fasta |
| Response: | 552 Transfer aborted. File too large |