Hi,
I’ve been attempting to extract the pairwise alignments from the human and zebrafish genomes using the “Extract Pairwise MAF Blocks” tool but had no success. A few years ago I was able complete this with hg19 and danRer5 and after reading another support ticket from Feb 2019 I assume hg19 is the assembly still indexed. Whenever I run the tool I am met with the error “The MAF source specified (PAIRWISE_hg19_danRer5) appears to be invalid”. I have attempted this with other assemblies and been met with the same error (eg human vs chimp and human vs mouse). Is there something I’m missing?
Cheers,
James.