Help to evaluate my assembly results

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Hello,

I preprocessed my FASTQ files using fastp and then used the flye tool for assembly. After that, I polished the data using BWA-MEM and ran Medaka for further polishing. I’m unsure about the quality of my assembly.

I need assistance evaluating my three types of assembly (see attached Quast output).

  1. Polished assembly by Medaka
  2. Polished assembly By BWA-MEM
  3. Assembly flye without polishing

It would be helpful for me to understand my assembly and offer any suggestions for improvement.

Thanks in advance.

Galaxy2586-Quast_on_data_2530,_data_2529,_and_others__Circos_plot
Galaxy2586-Quast_on_data_2530,_data_2529,_and_others__Circos_plot3000×3000 1.13 MB

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image1084×893 21.2 KB

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2

Hi @l.r.l.s.k
Have you looked at GTN tutorials, for example

Check questions and read the answers (click at Solution box to see answers).

Hope that helps.

Kind regards,
Igor

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