have been working through the 16S microbial analysis with mothur using my data. I have followed the tutorial on galaxy.eu. I was hoping to generate an ASV file instead of an OTU, and I was wondering how I could do that? I ran the pre.cluster tool, followed by removing chimeras and lineages. I used the make.shared tool but it required a list file that I do not have. I also tried to cluster tools to generate a list file but that only resulted in an error. How do I generate an asv table from the make.shared tool if I do not have a list file.
I am new to Bioinformatic and I am sorry to have to bother you with such questions.
I hope to hear from you soon.