Hi @pax
Troubleshooting options:
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Try comparing your inputs to the test data included in this prior Q&A. I understand that your data is in a collection, so check the fastq elements inside the collection and your tool form settings. Nanopore data assembly with CANU
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Make sure your collection is formatted correctly. All elements should have the same datatype assigned, and actually have content that matches that datatype. Collection tutorials: https://training.galaxyproject.org/training-material/topics/galaxy-data-manipulation/
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If the inputs are formatted correctly and the tool form settings match the content, the job could be failing for resources. Is this your own Galaxy server or some public Galaxy server? If public, what is the URL?
Let’s start there, thanks!