Consolidating into → Need Help with Galaxy LEfSe LDA Step Error
Let’s keep your question in a single topic instead of breaking up into so many repeated questions across topics.
You can clarify in that other topic about how you are “using the tool locally”. If this is in a Galaxy server, then the version of Galaxy you are running will be relevant along with how/where you installed the tool, then details about the tool version and usage I listed out there.
Thanks! 