I am learning the tutorial of Pre-processing of Single-Cell RNA Data
the UMI-tools extract on the tutorial is Galaxy version 0.5.5.1 but the current version is Galaxy Version 1.1.6+galaxy0. The following parameters are not presented on the current version of UMI-tools extract:
- “Barcode on both reads?”:
Barcode on first read only
- “Use Known Barcodes?”:
Yes
“Barcode File”*:celseq_barcodes.192.tabular
(Input dataset) - “Barcode pattern for first read”:
NNNNNNCCCCCC
- “Enable quality filter?”:
No
I put the following information on UMI-tools extract (Galaxy Version 1.1.6+galaxy0):
Library type Paired-end Dataset Collection
Reads in FASTQ format * 7: C57_P1_B1
Barcode pattern for first read * NNNNNNCCCCCC
Barcode Extraction Method String
Allowlist of accepted barcodes - optional: 9: celseq_barcodes.192.tabular
Enable quality filter? No
This returns 0 bytes in both forward and reverse reads
Would you help me to solve this problem?