Questions about UMI-tools extract Extract UMI from fastq files (Galaxy Version 1.1.6+galaxy0) in scRNAseq

1

I am learning the tutorial of Pre-processing of Single-Cell RNA Data
the UMI-tools extract on the tutorial is Galaxy version 0.5.5.1 but the current version is Galaxy Version 1.1.6+galaxy0. The following parameters are not presented on the current version of UMI-tools extract:

  • “Barcode on both reads?”: Barcode on first read only
  • “Use Known Barcodes?”: Yes
    “Barcode File”*: celseq_barcodes.192.tabular (Input dataset)
  • “Barcode pattern for first read”: NNNNNNCCCCCC
  • “Enable quality filter?”: No
    I put the following information on UMI-tools extract (Galaxy Version 1.1.6+galaxy0):
    Library type Paired-end Dataset Collection
    Reads in FASTQ format * 7: C57_P1_B1

Barcode pattern for first read * NNNNNNCCCCCC
Barcode Extraction Method String
Allowlist of accepted barcodes - optional: 9: celseq_barcodes.192.tabular
Enable quality filter? No

This returns 0 bytes in both forward and reverse reads
Would you help me to solve this problem?