Hi, I’m new to RNA seq and I’m trying to use blastx on galaxy to blast a fasta file of possibly novel lncRNAs using the ncbi nr database. I want to remove transcripts with significant homology to known proteins with: e-value < 1e-10, target coverage > 80%, and identity > 90%, so I can preserve tran…

@gallardoalba I tried it with choose remote file option and it worked perfectly

Use blastx on Galaxy

jennaj Split this topic September 21, 2022, 4:36pm 12

A post was split to a new topic: Troubleshooting use of FEELnc

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