I’m new to RNA seq and I’m trying to use blastx on galaxy to blast a fasta file of possibly novel lncRNAs using the ncbi nr database. I want to remove transcripts with significant homology to known proteins with: e-value < 1e-10, target coverage > 80%, and identity > 90%, so I can preserve transcripts that are most likely lncRNAs. I tried to change the e-value/expectation value to 0.0000000001 and query coverage to 80% but I cannot change the pident setting (percentage of identical matches). How do I do this? Also, when I run the tool on default settings it is running for more than a day.