Hi, I’m new to RNA seq and I’m trying to use blastx on galaxy to blast a fasta file of possibly novel lncRNAs using the ncbi nr database. I want to remove transcripts with significant homology to known proteins with: e-value < 1e-10, target coverage > 80%, and identity > 90%, so I can preserve tran…

@gallardoalba I tried it with choose remote file option and it worked perfectly

Use blastx on Galaxy

gallardoalba October 27, 2021, 9:05am 2

Hi @bart_joosten,
could you describe to me your pipeline until now? Do you have raw RNA-seq data or assembled transcripts? Which species do the samples correspond to?

Regards

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Use blastx on Galaxy

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