I’m hoping someone may be able to help. My advisor is attempting to use Galaxy for RNAseq analysis and he created a workflow for the alignment via Hisat2 and counts via feature count for a number of paired end samples. I double checked his workflow to make sure it worked correctly with some fastq files that I had in my own history. But, it’s not working on his end with his files, as follows:
Any ideas as to why this may be? Thanks in advance for your help.