Workflow Errors

Hello all,

I’m hoping someone may be able to help. My advisor is attempting to use Galaxy for RNAseq analysis and he created a workflow for the alignment via Hisat2 and counts via feature count for a number of paired end samples. I double checked his workflow to make sure it worked correctly with some fastq files that I had in my own history. But, it’s not working on his end with his files, as follows:

Any ideas as to why this may be? Thanks in advance for your help.

are you have this problem yet ? or it solved ?

Is he trying this on a different Galaxy instance, then the one you used to create the WF?
Are there any WF inputs further down the form (not in the screenshot) that he didn’t populate?