Hi all, Is there a way to do base calling in Galaxy? I think this is where fast5 files are concatenated to one big fastq file for downstream analysis?
Best wishes.
Hi @Martyn
Would this tool do what you want? Screenshot is from the UseGalaxy.eu server.
Tool → Extract FASTQ in tabular format from a set of FAST5 files
Resources
- Single Cell / Tutorial List
- Single Cell / Tutorial / Converting between common single cell data formats ← see the tabular to fastq manipulations in here for getting your data also transformed to fit your target protocol/tools