I am continuing the “Genomic Data Science” course from Coursera. However, I have to use Cufflink-Cuffmerge-Cuffdiff sequentially to perform differential expression analysis that existed last week. Cufflink and Cuffmerge work fine, but whatever I tried Cuffdiff failed. I could not understand why this is so.
BAM record error: found spliced alignment without XS attribute
I get this error line.