Replicates will always be required when running these tools in Galaxy. If you really need to do exploratory non-replicate analysis, then using the tools line-command is the standard alternative.
Apologies – but this requirement in the Galaxy-wrapped version of the tools was necessary to avoid wide-spread odd job errors (that were not only confusing to troubleshoot but draining shared public computational resources, often for no practical purpose) and even more importantly, to alert scientists about problematic experimental design even if/when putatively “successful” green results were produced.
We tried not requiring replicates originally, but very quickly realized it wasn’t working out well for the vast majority of scientists.