I am a plants.usegalaxy.eu/ user and my id is jvd.choudhary@gmail.com . I am working on miRNA differential expression, for that, I use the miRdeep2 mapper, miRdeep2 quantifier workflow and generated the quantifier tabular output, further I need to use the tool for differential expression like DeSeq2 …

How to calculate the differential expression without replicate data

gallardoalba November 17, 2021, 5:16pm 2

sorry, but Galaxy restricts edgeR to at least two replicates. You can find more information about this topic in the following post: DESeq2 or EdgeR or Limma without replication - #7 by jennaj

Regards

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