I have a RNA-seq have no replicates, so I am using edgeR to do DE. and I got counts data from featurecount. I run edgeR, but keep turning red… the warning message see below.
Could anyone help me with that?
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
In estimateDisp.default(y = y$counts, design = design, group = group, :
No residual df: setting dispersion to NA
Error in plotMDS.default(y, top = top, labels = labels, pch = pch, cex = cex, :
Only 2 columns of data: need at least 3
Calls: plotMDS → plotMDS.DGEList → plotMDS → plotMDS.default