Hi, I am running EdgeR and got following errors. Can you please instruct me on how to solve these issues
- Error in
.rowNamesDF<-
(x, value = value) :
duplicate ‘row.names’ are not allowed
Calls: row.names<- -> row.names<-.data.frame -> .rowNamesDF<-
Warning message:
non-unique values when setting ‘row.names’: ‘HISAT2.on.data.38…data.37…and.data.50…ali - Error in quantile.default(x, p = p) :
missing values and NaN’s not allowed if ‘na.rm’ is FALSE
Calls: calcNormFactors … .calcFactorQuantile -> apply -> FUN -> quantile -> quantile.default
Further, I got following message in DEseq2
- estimating size factors
Error in estimateSizeFactorsForMatrix(counts(object), locfunc = locfunc, :
every gene contains at least one zero, cannot compute log geometric means
Calls: DESeq … estimateSizeFactors -> .local -> estimateSizeFactorsForMatrix
I am in a really tight schedule. So that it will be great if I can get information asap.
Thank you
Randika Yapa