Error when performing Deseq2 analysis help

Hello,

I’m running into an issue with performing Deseq analysis. Has anyone encountered this error before and could help me troubleshoot?
Error in file(filename, “r”, encoding = encoding) :
cannot open the connection
Calls: source_local -> source -> file
Warning message:
In file(filename, “r”, encoding = encoding) :
cannot open file '/jetstream/scratch0/main/jobs/32794987/tool_files/g

Thank you!