DeSeq2 error, RNA-seq,


I am working RNA-seq dataset in influenza virus infection. I am doing differential expression using DeSeq2 in usegalaxy. I am keep getting this error message

Warning message:
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
Error in DESeqDataSet(se, design = design, ignoreRank) :

Could anyone please give me advice on how to resolve this? This is my first time analyze RNAseq


Hi @Vu_Ngo

This is a technical error.

First, try a rerun.

Next, check your inputs/parameters. Content and format both matter. The tool form has many details, including a link out to the Bioconductor forum. Galaxy tutorials and this forum are also good places to review. All can be searched with keywords like tool names.

If you cannot solve the problem and are working at a public Galaxy server, please share back the server URL and the full error report.

Let’s start there :slight_smile:

Hi @jennaj

Thank you so much. Following your advice, I was able to solve the problem.

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