When I used the Desq2 with featurecounts output files, this error occurred:
Fatal error: An undefined error occurred, please check your input carefully and contact your administrator.
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
Error in DESeqDataSet(se, design = design, ignoreRank) :
some values in assay are negative
Calls: get_deseq_dataset … DESeqDataSetFromHTSeqCount → DESeqDataSetFromMatrix → DESeqDataSet
I then used the data from the tutorial [Reference-based RNA-Seq data analysis], and same error showed up again.
Could anyone help me with this bug? Many thanks.