DESeq2 Fatal error In Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") :

When I used the Desq2 with featurecounts output files, this error occurred:

Fatal error: An undefined error occurred, please check your input carefully and contact your administrator.
Warning message:
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
Error in DESeqDataSet(se, design = design, ignoreRank) :
some values in assay are negative
Calls: get_deseq_dataset … DESeqDataSetFromHTSeqCount → DESeqDataSetFromMatrix → DESeqDataSet

I then used the data from the tutorial [Reference-based RNA-Seq data analysis], and same error showed up again.
Could anyone help me with this bug? Many thanks.

Hi @hands
the error message is somewhat unusual:
some values in assay are negative
Maybe sort the input files and check if negative values are present.
Have you changed any DESeq2 settings during the tutorial?
Can you share the history with the tutorial?
Kind regards,