Click on the job details icon for the error dataset
Then on the
stderr link inside the report. You’ll probably see something like this for the full error message:
uk.ac.babraham.FastQC.Sequence.SequenceFormatException: Ran out of data in the middle of a fastq entry. Your file is probably truncated
If that is included in your error, then the Upload of your data was not complete or the data was truncated before uploading to Galaxy or possibly malformed in the middle somewhere (from some upstream transfer or manipulation).
Try re-uploading the data first (use FTP if large), making sure it completely loaded, and see if that resolves the problem. If not, examine the data locally to see if truncated/malformed in the original and fix it before uploading to Galaxy.
We can troubleshoot more from there with help from the Galaxy EU team if needed.